Characterisation of Indonesian H5N1 isolates to understand the prolonged infection and spread of H5N1 among domestic poultry

Wibawa, Hendra, Nuradji, Harimurti, Henning, Joerg, Bingham, John, Wong, Frank, Lowther, Sue, Payne, Jean, Rookes, Jenni and Meers, Joanne (2010). Characterisation of Indonesian H5N1 isolates to understand the prolonged infection and spread of H5N1 among domestic poultry. In: Threats and Impacts Stream Abstracts. Global Biosecurity Conference, Brisbane, Australia, (). 28 February - 3 March 2010.

Author Wibawa, Hendra
Nuradji, Harimurti
Henning, Joerg
Bingham, John
Wong, Frank
Lowther, Sue
Payne, Jean
Rookes, Jenni
Meers, Joanne
Title of paper Characterisation of Indonesian H5N1 isolates to understand the prolonged infection and spread of H5N1 among domestic poultry
Conference name Global Biosecurity Conference
Conference location Brisbane, Australia
Conference dates 28 February - 3 March 2010
Proceedings title Threats and Impacts Stream Abstracts
Publication Year 2010
Sub-type Oral presentation
Language eng
Abstract/Summary Highly pathogenic avian influenza (HPAI) caused by H5N1 virus has caused devastating outbreaks of disease in poultry populations around the world and has also resulted in deaths in humans. Indonesia is one of the countries most severely affected by H5N1 virus in terms of both poultry and human health. Although many control measures including stamping-out, vaccination, biosecurity and surveillance, have been implemented in Indonesia since 2004, HPAI H5N1 outbreaks still occur frequently in traditional sectors of poultry management. Domestic ducks may be an important source of H5N1 virus in Indonesia. We are currently investigating the role of ducks in maintaining H5N1 infection and shedding virus over prolonged periods. In this work we aim to determine the genetic and antigenic characteristics of H5N1 viruses isolated from small-holder duck farms in Indonesia and to describe the pathogenicity of two of these duck-derived isolates in ducks and chickens. Over 100 virus isolates were obtained during a longitudinal field study and disease outbreak investigations in Central Java. The nucleotide sequences of haemagglutinin (HA) and neuraminidase (NA) genes of selected isolates were analysed and antigenic mapping using haemagglutination inhibition data was performed. Groups of 14 chickens and 14 ducks were challenged with virus isolates from H5N1 subclades 2.1.1. and 2.1.3 and the birds were monitored for clinical signs, virus shedding and tissue distribution of virus. Results of these studies will be presented. This experimental and molecular research will help to understand the 33 HPAI epidemiology we observe in the field and may lead to improved methods to prevent future outbreaks.
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: School of Veterinary Science Publications
 
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Created: Mon, 07 Mar 2011, 15:46:41 EST by Assoc Prof Joanne Meers on behalf of School of Veterinary Science