A plexiglass tube-type water profile sampler was designed for use in relatively shallow bodies of water, such as farm ponds, small recreational lakes, irrigation canals, and the surface 2 m of larger lakes and rivers. The sampler is a plexiglass tube with holes drilled at intervals (side ports) corresponding to water layers which are to be sampled. To obtain a water sample, the tube (open at both ends and with the side ports closed) was gently lowered vertically into the water. With the top stoppered, the tube was raised and its bottom stoppered just under the water surface. While the tube is held vertically, the water profile samples are rapidly and easily obtained after removing the top stopper and successively collecting from top to bottom the water sample from each side port. Dissolved oxygen (DO) profiles of the ponds measured with an oxygen probe directly in the ponds were similar to DO profiles of water samples collected with the tube sampler. Chemical and biological analyses of water samples obtained with the tube-type sampler and two other standard type water samplers (Kemmerer and Van Dorn) were compared. The analyses revealed that similar relative results were obtained from water samples collected with all three samplers, but the tube-type sampler was more desirable for obtaining water profile samples because of its simplicity of design and operation, especially in small ponds.