Free radical scavenging and cytoprotective activities of phenolic antioxidants

Zhang, Jingli, Stanley, Roger A., Adaim, Aselle, Meton, Laurence D. and Skinner, Margot A. (2006) Free radical scavenging and cytoprotective activities of phenolic antioxidants. Molecular Nutrition and Food Research, 50 11: 996-1005. doi:10.1002/mnfr.200600072

Author Zhang, Jingli
Stanley, Roger A.
Adaim, Aselle
Meton, Laurence D.
Skinner, Margot A.
Title Free radical scavenging and cytoprotective activities of phenolic antioxidants
Journal name Molecular Nutrition and Food Research   Check publisher's open access policy
ISSN 1613-4125
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1002/mnfr.200600072
Volume 50
Issue 11
Start page 996
End page 1005
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley - VCH Verlag
Language eng
Formatted abstract
The free radical scavenging activities of three flavonoids (quercetin, rutin and catechin) and four hydroxycinnamic acids (caffeic, ferulic, sinapic, and chlorogenic acids) were evaluated using both oxygen radical absorbance capacity (ORAC) and lipid peroxidation inhibition capacity (LPIC) assays. The cytoprotective effects of these compounds were also measured by the degree of protection against H2O2-induced damage of human Jurkat cells. All compounds exhibited protection against H2O2-mediated cytotoxicity in a dose-dependent manner. The concentrations required to result in a 50% reduction in cell death (EC50 value) were calculated from their dose-response curves. These ranged from 0.15–2.65 μM. Overall, the four hydroxycinnamic acids tested were less effective than the three flavonoids, and of all compounds tested, quercetin offered the strongest protection against H2O2-induced cell death. A comparison of the results showed that the ability to inhibit peroxidation of lipids in a liposomal system (LPIC) correlated well with the cytoprotective activities (EC50), but not with the ability to protect an aqueous fluorescent substrate in the ORAC assays. The results suggest that the behavior of antioxidants in a liposomal membrane is to some extent similar to the mechanism involved in the protection of living cells from oxidative damage.
Keyword Antioxidant
Hydroxycinnamic acids
Lipid peroxidation inhibition capacity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
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Created: Mon, 07 Mar 2011, 15:28:56 EST