An NMR assay for quantitating lipase activity in biphasic macroemulsions

O'Connor, C. J., Petricevic, S. F., Coddington, J. M. and Stanley, R. A. (1992) An NMR assay for quantitating lipase activity in biphasic macroemulsions. Journal of the American Oil Chemists' Society, 69 4: 295-300. doi:10.1007/BF02636053

Author O'Connor, C. J.
Petricevic, S. F.
Coddington, J. M.
Stanley, R. A.
Title An NMR assay for quantitating lipase activity in biphasic macroemulsions
Journal name Journal of the American Oil Chemists' Society   Check publisher's open access policy
ISSN 0003-021X
Publication date 1992-04
Sub-type Article (original research)
DOI 10.1007/BF02636053
Volume 69
Issue 4
Start page 295
End page 300
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract A novel nuclear magnetic resonance (NMR) assay has been developed to monitor lipase-catalyzed esterification reactions without the need to extract and purify the individual components. The technique measures ratios of ester:alcohol signals and has been shown to be both efficient and reproducible. The assay has proven useful as a quick screen for the effect of varying conditions on the extent of esterification in various biphasic solvent systems and can be applied to both saturated and unsaturated long-chain fatty acid and alcohol substrates. The NMR ratio technique has been used to quantitate the extent of reaction in the Candida cylindracea lipase-catalyzed synthesis of oleyl palmitate, stearyl palmitate, oleyl oleate, oleyl gamma-linolenate and oleyl linoleate. The identity of these products was confirmed by high-resolution mass spectrometry.
Keyword Candida-cylindracea
Ester synthesis
Lipase activity
Nmr assay
Positional specificity of enzyme reactions
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 07 Mar 2011, 15:27:07 EST