An NMR assay for quantitating lipase activity in biphasic macroemulsions

O'Connor, C. J., Petricevic, S. F., Coddington, J. M. and Stanley, R. A. (1992) An NMR assay for quantitating lipase activity in biphasic macroemulsions. Journal of the American Oil Chemists' Society, 69 4: 295-300.


Author O'Connor, C. J.
Petricevic, S. F.
Coddington, J. M.
Stanley, R. A.
Title An NMR assay for quantitating lipase activity in biphasic macroemulsions
Journal name Journal of the American Oil Chemists' Society  (ERA 2012 Listed)    (ERA 2010 Rank C)   Check publisher's open access policy
Publication date 1992-04
Sub-type Article
DOI 10.1007/BF02636053
Volume number 69
Issue number 4
ISSN 0003-021X; 1558-9331
Start page 295
End page 300
Total pages 6
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract A novel nuclear magnetic resonance (NMR) assay has been developed to monitor lipase-catalyzed esterification reactions without the need to extract and purify the individual components. The technique measures ratios of ester:alcohol signals and has been shown to be both efficient and reproducible. The assay has proven useful as a quick screen for the effect of varying conditions on the extent of esterification in various biphasic solvent systems and can be applied to both saturated and unsaturated long-chain fatty acid and alcohol substrates. The NMR ratio technique has been used to quantitate the extent of reaction in the Candida cylindracea lipase-catalyzed synthesis of oleyl palmitate, stearyl palmitate, oleyl oleate, oleyl gamma-linolenate and oleyl linoleate. The identity of these products was confirmed by high-resolution mass spectrometry.
Keyword Candida-cylindracea
Esterification
Ester synthesis
Lipase activity
Macroemulsions
Nmr assay
Positional specificity of enzyme reactions
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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