Characterization of Ustilago tritici and U. nuda on wheat and barley. II. RAPD analysis

Sharifnabi, B., Mitter, N. and Agarwal, D. K. (2003) Characterization of Ustilago tritici and U. nuda on wheat and barley. II. RAPD analysis. Mycotaxon, 86 87-97.

Author Sharifnabi, B.
Mitter, N.
Agarwal, D. K.
Title Characterization of Ustilago tritici and U. nuda on wheat and barley. II. RAPD analysis
Journal name Mycotaxon   Check publisher's open access policy
ISSN 0093-4666
Publication date 2003-04
Sub-type Article (original research)
Volume 86
Start page 87
End page 97
Total pages 11
Language eng
Abstract Monokaryotic haploid hyphae of fourteen isolates of Ustilago spp. pathogenic on wheat and barley were analyzed by random amplified polymorphic DNA analysis (RAPD) using twenty-five decamer primers. The results of the RAPD analyses showed that a high degree of variability exists among isolates of Ustilago tritici. Isolate Ut12 gave a distinct pattern and was separated from all others by UPGMA cluster analysis. Amplification products obtained with primers OPN5 and OPM6 were able to clearly separate U. nuda isolates from all the U. tritici isolates. The study highlights the fact that DNA polymorphism can be used to separate U. nuda from U. tritici, which could not be done on the basis of teliospore morphology.
Keyword Monokaryotic haploid hyphae
Loose smut
Dna analysis
Upgma cluster analysis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 15:16:40 EST