Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences

Higgins, C. M., Hall, R. M., Mitter, N., Cruickshank, A. and Dietzgen, R. G. (2004) Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences. Transgenic Research, 13 1: 59-67. doi:10.1023/B:TRAG.0000017166.29458.74


Author Higgins, C. M.
Hall, R. M.
Mitter, N.
Cruickshank, A.
Dietzgen, R. G.
Title Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences
Journal name Transgenic Research   Check publisher's open access policy
ISSN 0962-8819
Publication date 2004-02
Sub-type Article (original research)
DOI 10.1023/B:TRAG.0000017166.29458.74
Volume 13
Issue 1
Start page 59
End page 67
Total pages 9
Language eng
Abstract Peanut (Arachis hypogaea L.) lines exhibiting high levels of resistance to peanut stripe virus (PStV) were obtained following microprojectile bombardment of embryogenic callus derived from mature seeds. Fertile plants of the commercial cultivars Gajah and NC7 were regenerated following co-bombardment with the hygromycin resistance gene and one of two forms of the PStV coat protein (CP) gene, an untranslatable, full length sequence (CP2) or a translatable gene encoding a CP with an N-terminal truncation (CP4). High level resistance to PStV was observed for both transgenes when plants were challenged with the homologous virus isolate. The mechanism of resistance appears to be RNA-mediated, since plants carrying either the untranslatable CP2 or CP4 had no detectable protein expression, but were resistant or immune (no virus replication). Furthermore, highly resistant, but not susceptible CP2 TO plants contained transgene-specific small RNAs. These plants now provide important germplasm for peanut breeding, particularly in countries where PStV is endemic and poses a major constraint to peanut production.
Keyword Peanut
Potyvirus
Rna-mediated virus resistance
Transformation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 23 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 07 Mar 2011, 15:16:00 EST