Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum

Zhang, P., Blackall, P. J., Yamaguchi, T. and Iritani, Y. (2000) Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum. Veterinary Microbiology, 76 1: 91-101. doi:10.1016/S0378-1135(00)00224-8


Author Zhang, P.
Blackall, P. J.
Yamaguchi, T.
Iritani, Y.
Title Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2000-09-01
Sub-type Article (original research)
DOI 10.1016/S0378-1135(00)00224-8
Volume 76
Issue 1
Start page 91
End page 101
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
We report on the production and characterisation of monoclonal antibodies (MAbs) against Haemophilus paragallinarum, the causative agent of infectious coryza. A bank of 8 MAbs were produced by traditional techniques - four against the reference strain for Page serovar A (0083) and four against the reference strain for Page serovar C (Modesto). Seven of the eight MAbs were shown to be IgG1 with one being nontypable. None of the MAbs had HI activity and none gave any detectable reaction when examined by Western blotting. None of the MAbs gave a positive reaction in the indirect ELISA with any of the eight type strains of Pasteurella species or sub-species. None of our 8 MAbs gave serovar specific reactions when used in an indirect ELISA format. There was a trend for the serovar A MAbs to give a higher titre with serovar A isolates/strains and a similar trend for the serovar C MAbs to give higher titres with the serovar C isolates/strains.
Keyword Haemophilus paragallinarum
Monoclonal antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Tue, 08 Mar 2011, 00:46:24 EST