Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library

Wang, Hongjun, Gao, Yaping, Gong, Yumei, Chen, Xiaoling, Liu, Chuan, Zhou, Xuemei, Blackall, P. J., Zhang, Peijun and Yang, Hanchun (2007) Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Veterinary Microbiology, 119 2-4: 231-239.


Author Wang, Hongjun
Gao, Yaping
Gong, Yumei
Chen, Xiaoling
Liu, Chuan
Zhou, Xuemei
Blackall, P. J.
Zhang, Peijun
Yang, Hanchun
Title Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library
Formatted title Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2007-01-31
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2006.08.034
Volume 119
Issue 2-4
Start page 231
End page 239
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza.
Keyword Avibacterium paragallinarum
Mimotype
Immamogenicity
Phage display
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 17 September 2006

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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