An evaluation of the apxIVA based PCR-REA method for differentiation of Actinobacillus pleuropneumoniae

Turni, C. and Blackall, P. J. (2007) An evaluation of the apxIVA based PCR-REA method for differentiation of Actinobacillus pleuropneumoniae. Veterinary Microbiology, 121 1-2: 163-169.


Author Turni, C.
Blackall, P. J.
Title An evaluation of the apxIVA based PCR-REA method for differentiation of Actinobacillus pleuropneumoniae
Formatted title An evaluation of the apxIVA based PCR-REA method for differentiation of Actinobacillus pleuropneumoniae
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2007-03-31
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2006.11.016
Volume 121
Issue 1-2
Start page 163
End page 169
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract A restriction analysis of PCR (PCR-REA) amplified apxIVA gene has been suggested as an alternative method for serotyping of Actinobacillus pleuropneumoniae by Jaglic et al. [Jaglic, Z., Svastova, P., Rychlik, I., Nedbalcova, K., Kucerova, Z., Pavlik, I., Bartos, M., 2004. Differentiation of Actinobacillus pleuropneumoniae by PCR-REA based on sequence variability of the apxIVA gene and by ribotyping. Vet. Microbiol. 103, 63–69]. The current study investigated whether this alternative method could distinguish between the reference strains of serovars 13–15 and the value of the method when applied to 47 field isolates representing serovars 1–3, 5, 7–9, 12 and 15 as well as non-typable isolates. The reference strains of serovars 13 and 14 had the same sized product after the apxIVA PCR, while the product for serovar 15 was of different size compared to all the other serovar reference strains. The CfoI digest profiles of the reference serovars 13 and 14 strains were different from each other and from all other serovars. The HpaII digest profiles of these two serovars were very similar to each other, but both were distinctively different from the other serovar profiles. The CfoI digest profile of serovar 15 strain was very similar to the serovars 3 and 12 strains except for two faint extra bands for serovar 15. The HpaII digest profiles of serovars 12 and 15 reference strains were identical. The PCR-REA method correctly recognized the serovar of 21 of 43 field isolates. It was concluded that the method was a useful additional tool to support, but could not replace, conventional serotyping.
Keyword Actinobacillus pleuropneumoniae
ApxIVA
PCR-REA
CfoI
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Short communications. Available online 25 November 2006.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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