Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis

Turni, C. and Blackall, P. J. (2005) Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis. Veterinary Microbiology, 106 1-2: 145-151.


Author Turni, C.
Blackall, P. J.
Title Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis
Formatted title Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis
Journal name Veterinary Microbiology  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2005-03-20
Sub-type Article
DOI 10.1016/j.vetmic.2004.12.019
Volume number 106
Issue number 1-2
ISSN 0378-1135
1873-2542
Start page 145
End page 151
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract The aim of this study was to compare the use of indirect haemagglutination (IHA) and gel diffusion (GD) tests for serotyping Haemophilus parasuis by the Kielstein–Rapp-Gabrielson (KRG) scheme. All 15 serovar reference strains, 72 Australian field isolates, nine Chinese field isolates, and seven isolates from seven experimentally infected pigs were evaluated with both tests. With the IHA test, 14 of the 15 reference strains were correctly serotyped—with serovar 10 failing to give a titre with serovar 10 antiserum. In the GD test, 13 reference strains were correctly serotyped—with antigen from serovars 7 and 8 failing to react with any antiserum. The IHA methodology serotyped a total of 45 of 81 field isolates while the GD methodology serotyped a total of 48 isolates. For 29 isolates, the GD and IHA methods gave discordant results. It was concluded that the IHA is a good additional test for the serotyping of H. parasuis by the KRG scheme if the GD methodology fails to provide a result or shows unusual cross-reactions.
Keyword Haemophilus parasuis
Indirect haemagglutination
IHA
Gel diffusion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Short Communications

Document type: Journal Article
Sub-type: Article
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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