A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens

Sun, H., Miao, D., Zhang, P., Gong, Y. and Blackall, P. J. (2007) A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens. Biologicals, 35 4: 317-320. doi:10.1016/j.biologicals.2007.04.004


Author Sun, H.
Miao, D.
Zhang, P.
Gong, Y.
Blackall, P. J.
Title A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens
Formatted title A comparison of a blocking ELISA and a haernagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens
Journal name Biologicals   Check publisher's open access policy
ISSN 1045-1056
1095-8320
Publication date 2007-10
Sub-type Article (original research)
DOI 10.1016/j.biologicals.2007.04.004
Volume 35
Issue 4
Start page 317
End page 320
Total pages 4
Place of publication London, United Kingdom
Publisher Academic Press
Language eng
Formatted abstract The ability of blocking ELISAs and haemagglutination-inhibition (HI) tests to detect antibodies in sera from chickens challenged with either Avibacterium (Haemophilus) paragallinarum isolate Hp8 (serovar A) or H668 (serovar C) was compared. Serum samples were examined weekly over the 9 weeks following infection. The results showed that the positive rate of serovar A specific antibody in the B-ELISA remained at 100% from the second week to the ninth week. In chickens given the serovar C challenge, the highest positive rate of serovar C specific antibody in the B-ELISA appeared at the seventh week (60% positive) and was then followed by a rapid decrease. The B-ELISA gave significantly more positives at weeks 2, 3, 7, 8 and 9 post-infection for serovar A and at week 7 post-infection for serovar C. In qualitative terms, for both serovar A and serovar C infections, the HI tests gave a lower percentage of positive sera at all time points except at 9 weeks post-infection with serovar C. The highest positive rate for serovar A HI antibodies was 70% of sera at the fourth and fifth weeks post-infection. The highest rate of serovar C HI antibodies was 20% at the fifth and sixth weeks post-infection. The results have provided further evidence of the suitability of the serovar A and C B-ELISAs for the diagnosis of infectious coryza.
Keyword Blocking ELISA
HI
Avibacterium paragallinarum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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