Phenotypic and molecular characterization of V-factor (NAD)-independent haemophilus paragallinarum

Miflin, J. K., Horner, R. F., Blackall, P. J., Chen, X., Bishop, G. C., Morrow, C. J., Yamaguchi , T. and Iritani, Y. (1995) Phenotypic and molecular characterization of V-factor (NAD)-independent haemophilus paragallinarum. Avian Diseases, 39 2: 304-308. doi:10.2307/1591871

Author Miflin, J. K.
Horner, R. F.
Blackall, P. J.
Chen, X.
Bishop, G. C.
Morrow, C. J.
Yamaguchi , T.
Iritani, Y.
Title Phenotypic and molecular characterization of V-factor (NAD)-independent haemophilus paragallinarum
Journal name Avian Diseases   Check publisher's open access policy
ISSN 0005-2086
Publication date 1995-04
Sub-type Article (original research)
DOI 10.2307/1591871
Volume 39
Issue 2
Start page 304
End page 308
Total pages 5
Place of publication Jacksonville, FL, United States
Publisher American Association of Avian Pathologists
Language eng
Abstract In South Africa from early 1989 onward, strains of Haemophilus paragallinarum not requiring nicotinamide adenine dinucleotide (NAD) have been isolated from commercial chickens suffering from infectious coryza. Fifteen of these field isolates were characterized by biochemical typing, serotyping, restriction endonuclease analysis (REA), and ribotyping. The chosen isolates represented diversity in geographic location, time of disease outbreak, and type of flock. All were typical of the species in biochemical properties, except that they were NAD-independent, and all were Page serovar A. REA was performed with three enzymes: HindIII, HpaII, and SspI. All isolates gave identical REA profiles with all three enzymes. Ribotyping was performed using a probe that consisted of the plasmid pUC19 into which the 16S rRNA operon of H. paragallinarum had been inserted. All 15 isolates gave the same ribotyping profile using each of the three enzymes. As a group, the NAD-independent strains gave REA profiles and ribotypes that were very different from a range of classic South African strains isolated before 1989. Our results strongly suggest that the NAD-independent isolates are clonal in nature.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
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