Confirmation that PCR can be used to identify NAD-dependent and NAD-independent Haemophilus paragallinarum isolates

Miflin, J.K., Chen, X., Bragg, R.R., Welgemoed, J.M., Greyling, J.M., Horner, R.F. and Blackall, P.J. (1999) Confirmation that PCR can be used to identify NAD-dependent and NAD-independent Haemophilus paragallinarum isolates. Onderstepoort Journal of Veterinary Research, 66 1: 55-57.

Author Miflin, J.K.
Chen, X.
Bragg, R.R.
Welgemoed, J.M.
Greyling, J.M.
Horner, R.F.
Blackall, P.J.
Title Confirmation that PCR can be used to identify NAD-dependent and NAD-independent Haemophilus paragallinarum isolates
Journal name Onderstepoort Journal of Veterinary Research   Check publisher's open access policy
ISSN 0030-2465
2219-0635
Publication date 1999-03
Sub-type Article (original research)
Volume 66
Issue 1
Start page 55
End page 57
Total pages 3
Place of publication Onderstepoort, South Africa
Publisher Agricultural Research Council * Onderstepoort Veterinary Institute
Language eng
Abstract Seventy five bacteria tentatively identified as Haemophilus paragallinarum (the causative agent of infectious coryza), eight identified as Ornithobacterium rhinotracheale and 13 identified as NAD-independent Pasteurella species were isolated from chickens with respiratory infection in various provinces in South Africa. The isolates were characterized by conventional biochemical and serological methods. A polymerase chain reaction (PCR) assay specific for H. paragallinarum was used to identify the cultures directly from colonies. The PCR assay gave positive results for all isolates that were identified by conventional methods as H. paragallinarum, irrespective of whether they were nicotinamide adenine dinucleotide (NAD)-dependent (43 isolates) or NAD-independent (32 isolates).The eight isolates that were identified by conventional methods as O. rhinotracheale and the 13 isolates identified as various Pasteurella species gave negative results in the PCR assay. This study has demonstrated that colony PCR is a rapid method for uniquely identifying both NAD-dependent and NAD-independent strains of H. paragallinarum and distinguishing them from other bacteria, such as O. rhinotracheale and Pasteurella species.
Keyword Haemophilus paragallinarum
Polymerase chain reaction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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