Molecular characterization of isolates of Haemophilus paragallinarum from China by ribotyping

Miflin, J.K., Chen, X. and Blackall, P.J. (1997) Molecular characterization of isolates of Haemophilus paragallinarum from China by ribotyping. Avian Pathology, 26 1: 119-127. doi:10.1080/03079459708419199


Author Miflin, J.K.
Chen, X.
Blackall, P.J.
Title Molecular characterization of isolates of Haemophilus paragallinarum from China by ribotyping
Journal name Avian Pathology   Check publisher's open access policy
ISSN 0307-9457
1465-3338
Publication date 1997-03
Sub-type Article (original research)
DOI 10.1080/03079459708419199
Volume 26
Issue 1
Start page 119
End page 127
Total pages 9
Place of publication Essex, United Kingdom
Publisher Taylor & Francis
Language eng
Abstract Ribotyping profiles were determined for 12 Page serovar A isolates of Haemophilus paragallinarum from five outbreaks of infectious coryza in Hebei province in the People's Republic of China. Four enzymes were used to generate restriction digests of chromosomal DNA: HaeIII, HindIII, HpaII and SspI. The digests were probed with a digoxigenin-11-dUTP-labelled DNA probe produced by PCR amplification of the 16S rDNA of the type strain of H. paragallinarum. Only one profile was seen among the 12 isolates when using either HindIII or SspI. Three different ribotype profiles were seen with HpaII, while four different profiles were detected with HaeIII. Cluster analysis of the profiles generated by HpaII and HaeIII corresponded with the known epidemiological history of the isolates. These results are the first molecular characterization of isolates of H. paragallinarum from China and confirm the existence of genetic diversity in the H. paragallinarum population in that country.
Keyword Restriction endonuclease analysis
Ribosomal RNA genes
Pasteurella multocida
Chromosomal DNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:42:49 EST