Development of a 23S rRNA-based PCR assay for the identification of Pasteurella multocida

Miflin, J.K. and Blackall, P.J. (2001) Development of a 23S rRNA-based PCR assay for the identification of Pasteurella multocida. Letters in Applied Microbiology, 33 3: 216-221.


Author Miflin, J.K.
Blackall, P.J.
Title Development of a 23S rRNA-based PCR assay for the identification of Pasteurella multocida
Journal name Letters in Applied Microbiology   Check publisher's open access policy
Publication date 2001-09
Sub-type Article
DOI 10.1046/j.1472-765x.2001.00985.x
Volume number 33
Issue number 3
ISSN 0266-8254
1472-765X
Start page 216
End page 221
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract Aims:
The aim of this work was to develop a rapid diagnostic test for Pasteurella multocida.

Methods and Results:
A polymerase chain reaction (PCR) assay using primers derived from the 23S rRNA gene sequence of Past. multocida was developed. The PCR assay correctly identified all 144 isolates of Past. multocida tested, including type strains of the three subspecies as well as the reference strains for the Heddleston and Carter typing schemes. Of 20 closely related bacteria from the family Pasteurellaceae tested, only the type strains of Past. canis biovar 2 and Past. avium biovar 2 were positive. These two bacteria, formerly known as Bisgaard Taxon 13, are the closest phylogenetic relatives of Past. multocida based on 16S ribosomal rRNA. All phylogenetically unrelated avian and porcine organisms tested were negative.

Conclusions:
This PCR enables rapid identification of Past. multocida colonies from avian or porcine origin.

Significance and Impact of the Study:
Veterinary diagnostic laboratories can use this PCR to rapidly and accurately diagnose fowl cholera and porcine pasteurellosis.
Keyword Ribosomal RNA
Infectious coryza
Sequences
Phylogeny
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 20 December 2001.

Document type: Journal Article
Sub-type: Article
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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