Phenotypic and genotypic characterisation of Pasteurella multocida isolated from pigs at slaughter in New Zealand

Jamaludin, R., Blackall, P. J., Hansen, M. F., Humphrey, S. and Styles, M. (2005) Phenotypic and genotypic characterisation of Pasteurella multocida isolated from pigs at slaughter in New Zealand. New Zealand Veterinary Journal, 53 3: 203-207. doi:10.1080/00480169.2005.36505


Author Jamaludin, R.
Blackall, P. J.
Hansen, M. F.
Humphrey, S.
Styles, M.
Title Phenotypic and genotypic characterisation of Pasteurella multocida isolated from pigs at slaughter in New Zealand
Formatted title
Phenotypic and genotypic characterisation of Pasteurella multocida isolated from pigs at slaughter in New Zealand
Journal name New Zealand Veterinary Journal   Check publisher's open access policy
ISSN 0048-0169
1176-0710
1176-0702
Publication date 2005-06
Sub-type Article (original research)
DOI 10.1080/00480169.2005.36505
Volume 53
Issue 3
Start page 203
End page 207
Total pages 5
Place of publication Wellington, New Zealand
Publisher New Zealand Veterinary Association
Language eng
Formatted abstract
AIMS: To examine pigs at slaughter in New Zealand for the presence of Pasteurella multocida, and to determine for isolates, their biochemical profiles, somatic and capsular types, and the presence or absence of the HSB and toxA genes, associated with haemorrhagic septicaemia (HS) and progressive atrophic rhinitis (PAR), respectively.

METHODS: Swabs from 173 lungs, 158 palatine tonsils and 82 nasal passages of pigs at two abattoirs in New Zealand were cultured for P. multocida using conventional techniques, and isolated colonies were subjected to biochemical tests for identification of biovars. Somatic serotyping was conducted using an agar gel immunodiffusion (AGID) test. Polymerase chain reaction (PCR) assays were used to confirm phenotypic identification of colonies using species-specific primers, capsule type using serogroup-specific primers and multiplex PCR, and to test for the presence of HSB and toxA genes.

RESULTS: Pasteurella multocida was isolated from 11/173 (6.4%) lung, 32/158 (20.2%) palatine tonsil and 5/82 (6.1 %) nasal swab samples, a total of 48 isolates from 413 samples (11.6%). Isolation rates per farm ranged from 1–53% of tissue samples collected from pigs 5–6 months of age. On phenotypic characterisation, isolates were allocated to seven main biovars, viz 1, 2, 3, 5, 9, 12, and a dulcitol-negative variant of Biovar 8, the majority (30/48) being Biovar 3. Of the 42 isolates for which somatic serotyping was conducted, 10% were Serovar 1, 79% were Serovar 3, 2% were Serovar 6,1, 2% were Serovar 12, and 7% could not be typed. All 48 isolates were confirned as P. multocida using a species-specific PCR. In the capsular multiplex PCR, 92% of isolates were Capsular (Cap) type A, 2% were Cap D, and 6% could not be typed. None of the samples were positive for the HSB or toxA genes.

CONCLUSION: Serovars or capsular types of P. multocida associated with HS or PAR in pigs were not detected. Establishment of species-specific, capsular and toxin PCR assays allowed the rapid screening of isolates of P. multocida, while serotyping provided an additional tool for epidemiological and tracing purposes.
Keyword Pigs
Pasteurella multocida
Biovar
Somatic typing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:42:04 EST