Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza

Corney, B. G., Diallo, I. S., Wright, L., Hewitson, G., De Jong, A., Tolosa, X., Burrell, P., Duffy, P., Rodwell, B., Boyle, D. B. and Blackall, P. J. (2008) Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza. Avian Pathology, 37 6: 599-604. doi:10.1080/03079450802449139


Author Corney, B. G.
Diallo, I. S.
Wright, L.
Hewitson, G.
De Jong, A.
Tolosa, X.
Burrell, P.
Duffy, P.
Rodwell, B.
Boyle, D. B.
Blackall, P. J.
Title Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza
Formatted title
Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza
Journal name Avian Pathology   Check publisher's open access policy
ISSN 0307-9457
1465-3338
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1080/03079450802449139
Volume 37
Issue 6
Start page 599
End page 604
Total pages 6
Place of publication Colchester, Essex, United Kingdom
Publisher Taylor & Francis
Language eng
Formatted abstract
A 5′ Taq nuclease assay specific for Avibacterium paragallinarum was designed and optimized for use in diagnosing infectious coryza. The region chosen for assay design was one of known specificity for Av. paragallinarum. The assay detected Av. paragallinarum reference strains representing the three Page and the eight Kume serovars, and field isolates from diverse geographical locations. No cross-reactions were observed with other Avibacterium species, with other bacteria taxonomically related to Av. paragallinarum nor with bacteria and viruses likely to be present in swabs collected from suspected infectious coryza cases. The detection limit for the assay was 6 to 60 colony-forming units per reaction. Twenty-two out of 53 swabs collected from sick birds reacted in the 5′ Taq nuclease assay, whereas Av. paragallinarum was not isolated from any of the swabs. All of the 22 swabs yielded other bacteria in culture. The presence of Av. paragallinarum in the swabs was also demonstrated by sequencing, thereby confirming the ability of the assay to detect Av. paragallinarum in the presence of other bacteria. The ability to quantify bacterial load in the swabs using the 5′ Taq nuclease assay was demonstrated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:41:22 EST