Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland

Chinivasagam, H. N., Corney, B. G., Wright, L. L., Diallo, I. S. and Blackall, P. J. (2007) Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland. Journal of Applied Microbiology, 103 2: 418-426. doi:10.1111/j.1365-2672.2007.03275.x


Author Chinivasagam, H. N.
Corney, B. G.
Wright, L. L.
Diallo, I. S.
Blackall, P. J.
Title Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland
Formatted title Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
1365-2672
Publication date 2007-08
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.2007.03275.x
Volume 103
Issue 2
Start page 418
End page 426
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract Aims:  To investigate the occurrence and levels of Arcobacter spp. in pig effluent ponds and effluent-treated soil.
Methods and Results:  A Most Probable Number (MPN) method was developed to assess the levels of Arcobacter spp. in seven pig effluent ponds and six effluent-treated soils, immediately after effluent irrigation. Arcobacter spp. levels in the effluent ponds varied from 6·5 × 105 to 1·1 × 108 MPN 100 ml−1 and in freshly irrigated soils from 9·5 × 102 to 2·8 × 104 MPN g−1 in all piggery environments tested. Eighty-three Arcobacter isolates were subjected to an abbreviated phenotypic test scheme and examined using a multiplex polymerase chain reaction (PCR). The PCR identified 35% of these isolates as Arcobacter butzleri, 49% as Arcobacter cryaerophilus while 16% gave no band. All 13 nonreactive isolates were subjected to partial 16S rDNA sequencing and showed a high similarity (>99%) to Arcobacter cibarius.
Conclusions:A. butzleri, A. cryaerophilus and A. cibarius were isolated from both piggery effluent and effluent-irrigated soil, at levels suggestive of good survival in the effluent pond.
Significance and Impact of the Study:  This is the first study to provide quantitative information on Arcobacter spp. levels in piggery effluent and to associate A. cibarius with pigs and piggery effluent environments.
Keyword Arcobacter spp.
Effluent
MPN
Pigs
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:40:19 EST