Investigation and application of methods for enumerating heterotrophs and Escherichia coli in the air within piggery sheds

Chinivasagam, H. N. and Blackall, P. J. (2005) Investigation and application of methods for enumerating heterotrophs and Escherichia coli in the air within piggery sheds. Journal of Applied Microbiology, 98 5: 1137-1145. doi:10.1111/j.1365-2672.2005.02560.x


Author Chinivasagam, H. N.
Blackall, P. J.
Title Investigation and application of methods for enumerating heterotrophs and Escherichia coli in the air within piggery sheds
Formatted title
Investigation and application of methods for enumerating heterotrophs and Escherichia coli in the air within piggery sheds
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
1365-2672
Publication date 2005-05
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.2005.02560.x
Volume 98
Issue 5
Start page 1137
End page 1145
Total pages 9
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Aims: To investigate methods for the recovery of airborne bacteria within pig sheds and to then use the appropriate methods to determine the levels of heterotrophs and Escherichia coli in the air within sheds.
Methods and Results: AGI-30 impingers and a six-stage Andersen multi-stage sampler (AMS) were used for the collection of aerosols. Betaine and catalase were added to impinger collection fluid and the agar plates used in the AMS. Suitable media for enumerating E. coli with the Andersen sampler were also evaluated. The addition of betaine and catalase gave no marked increase in the recovery of heterotrophs or E. coli. No marked differences were found in the media used for enumeration of E. coli. The levels of heterotrophs and E. coli in three piggeries, during normal pig activities, were 2·2 x 105 and 21 CFU m-3 respectively.
Conclusions: The failure of the additives to improve the recovery of either heterotrophs or E. coli suggests that these organisms are not stressed in the piggery environment. The levels of heterotrophs in the air inside the three Queensland piggeries investigated are consistent with those previously reported in other studies. Flushing with ponded effluent had no marked or consistent effect on the heterotroph or E. coli levels.
Significance and Impact of the Study: Our work suggests that levels of airborne heterotrophs and E. coli inside pig sheds have no strong link with effluent flushing. It would seem unlikely that any single management activity within a pig shed has a dominant influence on levels of airborne heterotrophs and E. coli.
Keyword Aerosols
AGI-30
Andersen sampler
Escherichia coli
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 10 MAR 2005

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
ERA 2012 Admin Only
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 07 Mar 2011, 14:40:04 EST