Further studies on the use of a polymerase chain reaction test for the diagnosis of infectious coryza

Chen, X., Song, C., Gong, Y. and Blackall, P.J. (1998) Further studies on the use of a polymerase chain reaction test for the diagnosis of infectious coryza. Avian Pathology, 27 6: 618-624.


Author Chen, X.
Song, C.
Gong, Y.
Blackall, P.J.
Title Further studies on the use of a polymerase chain reaction test for the diagnosis of infectious coryza
Journal name Avian Pathology   Check publisher's open access policy
ISSN 0307-9457
1465-3338
Publication date 1998-12
Sub-type Article (original research)
DOI 10.1080/03079459808419393
Volume 27
Issue 6
Start page 618
End page 624
Total pages 7
Place of publication Essex, United Kingdom
Publisher Taylor & Francis
Language eng
Abstract Further information is reported on the use of a polymerase chain reaction (PCR) test for the diagnosis of infectious coryza in China. The majority of sinus swabs taken from artificially infected chickens and stored in glycerol-enriched phosphate-buffered saline were still positive by PCR after storage for 180 days at either 4°C or -20°C. Storage of swabs in either saline or nutrient broth was not as effective. Traditional culture failed to detect H. paragallinarum after storage for 3 days, regardless of storage medium or storage temperature. With dry swabs, the PCR could detect H. paragallinarum after storage for 7 days or longer at either 4°C or -20°C, while traditional culture could not. In PCR tests on 64 artificially-challenged chickens, all were positive by PCR at the six sampling dates up to 18 days post-challenge. Traditional culture gave a similar result. Both PCR and culture detected 50% or less of chickens as positive at 21 and 24 days post challenge. Antibiotic treatment reduced the ability of both culture and the PCR test to detect H. paragallinarum. The value of the PCR test and its superiority over traditional culture for the diagnosis of infectious coryza has been confirmed in these experiments.
Keyword Haemophilus paragallinarum
Chickens
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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