Analysis of Haemophilus parasuis by multilocus enzyme electrophoresis

Blackall, P.J., Trott, D.J., Rapp-Gabrielson, V. and Hampson, D.J. (1997) Analysis of Haemophilus parasuis by multilocus enzyme electrophoresis. Veterinary Microbiology, 56 1-2: 125-134. doi:10.1016/S0378-1135(96)01342-9

Author Blackall, P.J.
Trott, D.J.
Rapp-Gabrielson, V.
Hampson, D.J.
Title Analysis of Haemophilus parasuis by multilocus enzyme electrophoresis
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
Publication date 1997-05
Sub-type Article (original research)
DOI 10.1016/S0378-1135(96)01342-9
Volume 56
Issue 1-2
Start page 125
End page 134
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract The diversity among 40 Australian isolates and eight reference strains of Haemophilus parasuis was examined using multilocus enzyme electrophoresis. Thirty-four electrophoretic types (ETs) were recognized with a mean diversity per locus of 0.405. One Australian isolate was located in an ET separated by a considerable distance (> 0.8) from the rest of the isolates, suggesting that it may represent a different species or subspecies. The remaining 33 ETs formed two distinct divisions (A and B), separated from each other by a distance of 0.506. All 12 Australian isolates of serovar 5 plus the two reference strains for this serovar were included in Division A. The only other isolates present in this Division were Australian isolates of serovars 4 and 13 and two nontypeable isolates. Division B contained a diverse range of serovars Australian isolates of serovars 1, 2, 7/10, 9 and 13 as well as the reference strains for serovars 1, 2, 3, 4, 8 and 9. These results supported other studies which demonstrated considerable diversity, and indicate that the population of H. parasuis may contain more than a single species or subspecies. There was considerable diversity even amongst isolates of the same serovar, indicating that serotyping is not a particularly suitable technique for strain typing in epidemiological studies.
Keyword Haemophilus parasuis
Multilocus enzyme electrophoresis
Genetic diversity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
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Created: Mon, 07 Mar 2011, 14:39:07 EST