Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms

Blackall, P. J., Pahoff, J. L., Marks, D., Fegan, N. and Morrow, C. J. (1995) Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms. Australian Veterinary Journal, 72 4: 135-138. doi:10.1111/j.1751-0813.1995.tb15033.x


Author Blackall, P. J.
Pahoff, J. L.
Marks, D.
Fegan, N.
Morrow, C. J.
Title Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
Publication date 1995-04
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.1995.tb15033.x
Volume 72
Issue 4
Start page 135
End page 138
Total pages 4
Place of publication Oxford United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Biochemical profiles, restriction endonuclease analysis (REA) and ribotyping were used to investigate Pasteurella multocida isolates from outbreaks of fowl cholera on 7 turkey farms in New South Wales. While only a single isolate was available from 5 of the farms, multiple isolates, 4 and 12 respectively, were available from the other 2 farms. The available field evidence suggested that 8 outbreaks had occurred with one farm suffering 2 outbreaks. The isolates obtained were all confirmed as Pasteurella multocida. Biochemical profiles allocated the isolates to 4 groups, 3 being variants of P multocida subsp multocida and the fourth being P multocida subsp septica. REA performed with HpaII established 7 groups. Ribotyping using the HpaII digests probed with the 16S rRNA operon of Haemophilus paragallinarum recognised the same 7 groups as REA. Unlike the biochemical profiles, both REA and ribotyping provided a fine subdivision that identified outbreaks as either related or unrelated. The REA and ribotyping patterns as well as biochemical profiles were stable for all isolates from the outbreaks in which multiple isolates were obtained from either the same bird or from different birds. REA and ribotyping were found to be superior to biotyping methods for the investigation of fowl cholera outbreaks
Keyword Bacterial Dna
Deoxyribonucleases
Animal disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Mon, 07 Mar 2011, 14:38:41 EST