Population structure and diversity of avian isolates of Pasteurella multocida from Australia

Blackall, Patrick J., Fegan, Narelle, Chew, Gerard T. I. and Hampson, David J. (1998) Population structure and diversity of avian isolates of Pasteurella multocida from Australia. Microbiology, 144 279-289. doi:10.1099/00221287-144-2-279


Author Blackall, Patrick J.
Fegan, Narelle
Chew, Gerard T. I.
Hampson, David J.
Title Population structure and diversity of avian isolates of Pasteurella multocida from Australia
Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
1465-2080
Publication date 1998-02
Sub-type Article (original research)
DOI 10.1099/00221287-144-2-279
Volume 144
Start page 279
End page 289
Total pages 11
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Formatted abstract
A total of 110 isolates of Pasteurella multocida from Australian poultry and reference strains for the 16 somatic serovars plus the three subspecies (gallicida, multocida, septica) were analysed to examine their population structure and diversity. The 81 field isolates examined by multilocus enzyme electrophoresis (MLEE) were diverse, being divided into 56 electrophoretic types (ETs), with the 19 reference strains in another 15 ETs. The population was clonal and somatic serotyping was not particularly useful in establishing relationships between isolates. The 71 ETs formed three distinct subclusters (A, B and C) at a genetic distance of 0.36. Biovars tended to be associated with these subclusters: A with biovars 1, 3, 4, 5 and 8 and B with biovars 2, 6, 7, 9 and 10. Ribotyping, performed on all 110 isolates using Hpall, recognized 21 ribotypes forming nine clusters (R1-R9). The isolates in ribotype cluster R1 were almost identical to those in MLEE cluster B. Using both MLEE and ribotyping, the 19 non-Australian reference strains were found to be distributed over the full diversity of the Australian isolates of P. multocida. This study has shown that a range of P. multocida clones are associated with fowl cholera in Australia and that many of the Australian isolates are similar to non-Australian reference strains. Both the MLEE results and the ribotyping data identified a previously unrecognized subset of P. multocida strains.
Keyword Pasteurella multocida
Multilocus enzyme electrophoresis
Ribotype
Genetic diversity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:37:56 EST