Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping

Blackall, P. J., Christensen, J. P. and Bisgaard, M. (1998) Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping. Australian Veterinary Journal, 76 6: 423-425. doi:10.1111/j.1751-0813.1998.tb12394.x


Author Blackall, P. J.
Christensen, J. P.
Bisgaard, M.
Title Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 1998-06
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.1998.tb12394.x
Volume 76
Issue 6
Start page 423
End page 425
Total pages 3
Place of publication Oxford United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective: The objective of this work was to examine the diversity within Australian isolates of Actinobacillus equuli and related organisms by the genotypic method of ribotyping.
Design:
Ribotyping, performed using the enzyme Haelll, was used to examine the diversity in 12 field isolates of A equuli (five being capable of fermenting L-arabinose), one field isolate of Pasteurella caballi and two unclassifiable field isolates. Isolates were obtained from Australian horses, except for three isolates of A equuli (one L-arabinose positive and two L-arabinose negative) which were obtained from horses and a pig in Africa. In addition, the type strains for A equuli and P caballi and a reference strain for Bisgaard Taxon 9 were included in the study.
Results: The ribotype patterns were analysed by computerised cluster analysis, yielding five clusters (A to E). All five of the L-arabinose positive A equuli were assigned to cluster A, with all the other seven A equuli isolates (all L-arabinose negative) and the type strain being assigned to cluster B. One of the two unclassified isolates formed cluster C along with the reference strain for Bisgaard Taxon 9. The remaining unclassified isolate formed cluster D. Cluster E consisted of the field isolate and reference strain of P caballi.
Conclusion:
The results of this study indicate that A equuli is a diverse species, with L-arabinose positive isolates of A equuli being quite distinct from typical L-arabinose negative isolates. Ribotyping appears to be a useful tool in confirming the identity of A equuli-like organisms from horses.
Keyword Actinobacillus equuli
Ribotyping
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Mon, 07 Mar 2011, 14:37:36 EST