Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation

Agranovski, Victoria, Ristovski, Zoran, Hargreaves, Megan, Blackall, Patrick J. and Morawska, Lidia (2003) Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation. Journal of Aerosol Science, 34 3: 301-317. doi:10.1016/S0021-8502(02)00181-7

Author Agranovski, Victoria
Ristovski, Zoran
Hargreaves, Megan
Blackall, Patrick J.
Morawska, Lidia
Title Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation
Journal name Journal of Aerosol Science   Check publisher's open access policy
ISSN 0021-8502
Publication date 2003-03
Sub-type Article (original research)
DOI 10.1016/S0021-8502(02)00181-7
Volume 34
Issue 3
Start page 301
End page 317
Total pages 17
Place of publication Oxford, United Kingdom
Publisher Pergamon
Language eng
Formatted abstract
This paper presents the results of the laboratory evaluation of the Ultraviolet Aerodynamic Particle Size Spectrometer, the novel instrument for real-time continues monitoring of bioaerosols. The main focus of this study was on evaluating selectivity, sensitivity, counting efficiency, and the detection limits of the UVAPS. The tests were performed with two types of aerosols, bacterial (e.g., Bacillus subtilis spores or vegetative cells, and Pseudomonas fluorescens) and non-bacterial (e.g., NaCl, latex, peptone water, and nutrient agar/broth). To control viability of airborne bacteria, the bioaerosols were simultaneously sampled with the AGI-30 impingers. The study has demonstrated the UVAPS cross-sensitivity to the non-bacterial organic materials and the need for careful preparation (washing) of test bacteria, in order to avoid the interference with the fluorescence signals of nutrient media used to grow bacteria. The results were indicative of strong sensitivity of the UVAPS to the type of airborne bacteria. The limitations in the capability of the UVAPS to measure bacterial spores were also found. Counting efficiency of the fluorescent particles was shown to depend on particle concentration with the upper limit of detection of the UVAPS approximately 6×107 particles/m3.
Keyword Bacterial aerosols
Real-time monitoring
Intrinsic fluorescence
Performance evaluation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
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Created: Mon, 07 Mar 2011, 14:36:28 EST