Performance evaluation of the UVAPS: Influence of physiological age of airborne bacteria and bacterial stress

Agranovski, V., Ristovski, Z., Hargreaves, M., Blackall, P. J. and Morawska, L. (2003) Performance evaluation of the UVAPS: Influence of physiological age of airborne bacteria and bacterial stress. Journal of Aerosol Science, 34 12: 1711-1727. doi:10.1016/S0021-8502(03)00191-5


Author Agranovski, V.
Ristovski, Z.
Hargreaves, M.
Blackall, P. J.
Morawska, L.
Title Performance evaluation of the UVAPS: Influence of physiological age of airborne bacteria and bacterial stress
Journal name Journal of Aerosol Science   Check publisher's open access policy
ISSN 0021-8502
1879-1964
Publication date 2003-12
Sub-type Article (original research)
DOI 10.1016/S0021-8502(03)00191-5
Volume 34
Issue 12
Start page 1711
End page 1727
Total pages 17
Place of publication Oxford, United Kingdom
Publisher Pergamon
Language eng
Abstract This study evaluated the effect of bacterial physiology, such as physiological age and stress, on the performance of the ultraviolet aerodynamic particle sizer (UV-APS, model 3312, TSI Inc., St. Paul, MN). Intensity of the fluorescent signals was measured for three bacteria having various sensitivities to environmental stresses, Bacillus subtilus (spores and vegetative cells), Pseudomonas fluorescens, and Micrococcus luteus. The performance of the UVAPS was found to depend on the type of airborne bacteria. In addition, the fluorescence signals for stationary-phase bacteria were generally stronger than for their log-phase counterparts. These results indicated that bacterial injury due to environmental stresses has a strong influence on the measured fluorescence signals. This hypothesis was confirmed by obtaining a linear relationship between the percentage of fluorescent particles and the proportion of injured bacteria in the total population of cultivable bacteria in samples simultaneously collected with the AGI-30 impingers. This indicates that the amount of fluorophors (specifically NADH) within injured bacteria is below the UVAPS sensitivity level. The practical implications of these findings are discussed in the paper. The reported results contribute to broadening our understanding of the method and may assist in developing sampling strategies for the application of the UVAPS to various bioaerosol studies.
Keyword Bacterial aerosols
Real-time monitoring
Intrinsic fluorescence
Performance evaluation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 07 Mar 2011, 14:36:16 EST