Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis

Singh, Davinder Pal, Filardo, Fiona F., Storey, Richard, Jermakow, Angelica M., Yamaguchi, Shinjiro and Swain, Stephen M. (2010) Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis. Physiologia Plantarum, 138 1: 74-90. doi:10.1111/j.1399-3054.2009.01289.x


Author Singh, Davinder Pal
Filardo, Fiona F.
Storey, Richard
Jermakow, Angelica M.
Yamaguchi, Shinjiro
Swain, Stephen M.
Title Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis
Formatted title
Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis
Journal name Physiologia Plantarum   Check publisher's open access policy
ISSN 0031-9317
Publication date 2010-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1399-3054.2009.01289.x
Volume 138
Issue 1
Start page 74
End page 90
Total pages 17
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
We have examined the role of gibberellins (GAs) in plant development by expression of the pea GA 2-oxidase2 (PsGA2ox2) cDNA, which encodes a GA inactivating enzyme, under the control of the MEDEA (MEA) promoter. Expression of MEA:PsGA2ox2 in Arabidopsis caused seed abortion, demonstrating that active GAs in the endosperm are essential for normal seed development. MEA:PsGA2ox2 plants had reduced ovule number per ovary and exhibited defects in phyllotaxy and leaf morphology which were partly suppressed by GA treatment. The leaf architecture and phyllotaxy defects of MEA:PsGA2ox2 plants were also restored by sly1-d which reduces DELLA protein stability to increase GA response. MEA:PsGA2ox2 seedlings had increased expression of the KNOTTED1-like homeobox (KNOX) genes, BP, KNAT2 and KNAT6, which are known to control plant architecture. The expression of KNOX genes is also altered in wild-type plants treated with GA. These results support the conclusion that GAs can suppress the effects of elevated KNOX gene expression, and raise the possibility that localized changes in GA levels caused by PsGA2ox2 alter the expression of KNOX genes to modify plant architecture.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Article first published online: 8 SEP 2009

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 07 Mar 2011, 11:15:44 EST by Dr Fiona Filardo on behalf of Aust Institute for Bioengineering & Nanotechnology