Screening of the HLDA9 panel on peripheral blood dendritic cell populations

Ding, Yitian, Ju, Xinsheng, Azlan, Maryam, Hart, Derek N. J. and Clark, Georgina J. (2011). Screening of the HLDA9 panel on peripheral blood dendritic cell populations. In: Pablo Engel and A. Santoni, Proceedings of the 9th International Workshop on Human Leukocyte Differentiation Antigens. The 9th International Conference on Human Leukocyte Differentiation Antigens (HLDA9), Barcelona, Spain, (161-166). 11-13 March 2010. doi:10.1016/j.imlet.2010.10.010


Author Ding, Yitian
Ju, Xinsheng
Azlan, Maryam
Hart, Derek N. J.
Clark, Georgina J.
Title of paper Screening of the HLDA9 panel on peripheral blood dendritic cell populations
Conference name The 9th International Conference on Human Leukocyte Differentiation Antigens (HLDA9)
Conference location Barcelona, Spain
Conference dates 11-13 March 2010
Proceedings title Proceedings of the 9th International Workshop on Human Leukocyte Differentiation Antigens   Check publisher's open access policy
Journal name Immunology Letters   Check publisher's open access policy
Place of Publication Amsterdam, Netherlands
Publisher Elsevier BV
Publication Year 2011
Year available 2010
Sub-type Fully published paper
DOI 10.1016/j.imlet.2010.10.010
ISSN 0165-2478
1879-0542
Editor Pablo Engel
A. Santoni
Volume 134
Issue 2
Start page 161
End page 166
Total pages 6
Collection year 2011
Language eng
Formatted Abstract/Summary
Dendritic cells (DC) are a heterogeneous population of bone marrow derived leucocytes that are essential in the initiation of primary T lymphocyte responses. DC are identified as Lineage negative, HLA-DR+ blood cells that can be further subdivided by CD11c to distinguish CD11c+ DC and the CD11c plasmacytoid DC. Plasmacytoid DC are the primary IFNα producing cells and express CD303, CD304 and CD123. The CD11c+ myeloid DC can be divided into populations by CD1c, CD16 and CD141 expression. Despite DC being a functionally unique population, they share many cell surface antigens with myeloid lineage cells and B lymphocytes. We used flow cytometry to screen fresh human blood DC populations with the HLDA9 panel of 63 directly labelled mAb which included mAb specific for a number of B lymphocyte antigens. Of this panel, 23 mAb did not bind LinHLA-DR+ DC and 10 bound all four populations. Eight mAb bound to the three CD11c+ DC populations whilst no mAb tested bound to only pDC. Some of the mAb expected to bind to DC populations failed in this analysis. Overall, this screening highlighted similarities between the CD11c+ DC subsets and the relatively immature state of peripheral blood DC.
© 2010 Elsevier B.V. All rights reserved.
Keyword Blood dendritic cells
Cell surface molecules
Antigens
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 21 October 2010

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 06 Mar 2011, 00:12:30 EST