Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex

Horwood, Paul Francis and Mahony, Timothy John (2011) Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. Journal of Virological Methods, 171 2: 360-363. doi:10.1016/j.jviromet.2010.11.020


Author Horwood, Paul Francis
Mahony, Timothy John
Title Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
1879-0984
Publication date 2011-02
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.jviromet.2010.11.020
Volume 171
Issue 2
Start page 360
End page 363
Total pages 4
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Abstract Bovine respiratory disease complex (BRDC) is considered the most significant illness associated with feedlot cattle in North America and possibly worldwide. BRDC is a multi-factorial disease with environmental conditions interacting with multiple viral and bacterial pathogens to produce severe respiratory illness. Bovine herpesvirus 1, bovine viral diarrhoea virus and bovine parainfluenza virus 3 are three of the major viruses associated with BRDC. In this study, a multiplex real-time RT-PCR using Taqman primers and probes was developed to detect simultaneously all three of these important BRDC viruses. The assay was optimised and validated using cell-culture infected material and bovine clinical samples from BRDC cases. The sensitivity of the assay was analysed by comparing the multiplex with the individual singleplex assays, which demonstrated that viral detection was not inhibited, by multiplexing the real-time RT-PCRs. This technique is the first reported real-time PCR method for BPIV-3 and the first multiplex designed to detect three BRDC viral pathogens.
Keyword Real-time PCR
Bovine respiratory disease complex
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 30 November 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Sun, 06 Mar 2011, 00:07:33 EST