Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress

Rosic, Nedeljka N., Pernice, Mathieu, Rodriguez-Lanetty, Mauricio and Hoegh-Guldberg, Ove (2011) Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress. Marine Biotechnology, 13 3: 355-365. doi:10.1007/s10126-010-9308-9


Author Rosic, Nedeljka N.
Pernice, Mathieu
Rodriguez-Lanetty, Mauricio
Hoegh-Guldberg, Ove
Title Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress
Formatted title
Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress
Journal name Marine Biotechnology   Check publisher's open access policy
ISSN 1436-2228
1436-2236
1053-6426
0941-2905
Publication date 2011-06
Year available 2010
Sub-type Article (original research)
DOI 10.1007/s10126-010-9308-9
Volume 13
Issue 3
Start page 355
End page 365
Total pages 12
Editor J. G. Burgess
Z. J. Liu
N. Fusetani
Place of publication New York, NY, U.S.A.
Publisher Springer New York
Collection year 2011
Language eng
Formatted abstract
Unicellular photosynthetic algae (dinoflagellate) from the genus Symbiodinium live in mutualistic symbiosis with reef-building corals. Cultured Symbiodinium sp. (clade C) were exposed to a range of environmental stresses that included elevated temperatures (29°C and 32°C) under high (100 μmol quanta m−2 s−1 Photosynthetic Active Radiation) and low (10 μmol quanta m−2 s−1) irradiances. Using real-time RT-PCR the stability of expression for the nine selected putative housekeeping genes (HKGs) was tested. The most stable expression pattern was identified for cyclophilin and S-adenosyl methionine synthetase (SAM) followed by S4 ribosomal protein (Rp-S4), Calmodulin (Cal), and Cytochrome oxidase subunit 1 (Cox), respectively. Thermal stress alone resulted in the highest expression stability for Rp-S4 and SAM, with a minimum of two reference genes required for data normalization. For Symbiodinium exposed to both, light and thermal stresses, at least five reference genes were recommended by geNorm analysis. In parallel, the expression of Hsp90 for Symbiodinium in culture and in symbiosis within coral host (Acropora millepora) was evaluated using the most stable HKGs. Our results revealed a drop in Hsp90 expression after an 18 h-period and a 24 h-period of exposure to elevated temperatures indicating the similar Hsp90 expression profile in symbiotic and non-symbiotic environments. This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates.
© Springer Science+Business Media, LLC 2010
Keyword Housekeeping genes
Real-time RT-PCR
Gene expression
Dinoflagellate
Climate change
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 29 July 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Global Change Institute Publications
Official 2011 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Mar 2011, 15:03:25 EST by Nicola De Silva on behalf of Global Change Institute