Analysis of Newcastle diseases virus quasispecies and factors affecting the emergence of virulent virus

Kattenbelt, Jacqueline A., Stevens, Matthew P., Selleck, Paul W. and Gould, Allan R. (2010) Analysis of Newcastle diseases virus quasispecies and factors affecting the emergence of virulent virus. Archives of Virology, 155 10: 1607-1615. doi:10.1007/s00705-010-0739-4


Author Kattenbelt, Jacqueline A.
Stevens, Matthew P.
Selleck, Paul W.
Gould, Allan R.
Title Analysis of Newcastle diseases virus quasispecies and factors affecting the emergence of virulent virus
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 2010-10-01
Year available 2010
Sub-type Article (original research)
DOI 10.1007/s00705-010-0739-4
Volume 155
Issue 10
Start page 1607
End page 1615
Total pages 9
Editor M.H.V. Regenmortel
Place of publication Austria
Publisher Springer Wien
Collection year 2011
Language eng
Abstract Genome sequence analysis of a number of avirulent field isolates of Newcastle disease virus revealed the presence of viruses (within their quasispecies) that contained virulent FO sequences. Detection of these virulent sequences below the -1 % level, using standard cloning and sequence analysis, proved difficult, and thus a more sensitive reverse-transcription realtime PCR procedure was developed to detect both virulent and avirulent NDV FO sequences. Reverse-transcription real-time PCR analysis of the quasispecies of a number of Newcastle disease virus field isolates, revealed variable ratios (approximately 1:4-1 :4,000) of virulent to avirulent viral FO sequences. Since the ratios of these sequences generally remained constant in the quasispecies population during replication, factors that could affect the balance of virulent to avirulent sequences during viral infection of birds were investigated. It was shown both in vitro and in vivo that virulent virus present in the quasi species did not emerge from the "avirulent background" unless a direct selection pressure was placed on the quasispecies, either by growth conditions or by transient immunosuppression. The effect of a prior infection of the host by infectious bronchitis virus or infectious bursal disease virus on the subsequent emergence of virulent Newcastle disease virus was examined.
Keyword Newcastle disease virus
Avirulent field isolates
Quasispecies
Virulent Virus
Pcr Analysis
Reverse-transcription
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Clinical Medical Virology Centre Publications
 
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Created: Wed, 02 Mar 2011, 23:42:55 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre