Long distance regulation of plant stem cell growth during legume nodulation

Gresshoff P.M., Kereszt, A., Indrasumunar, A., Nontachaiyapoom, S., Lin, Y. H., Jiang, Q., Miyagi, M., Oelkers, K., Mathesius, U., Lin, M., Li, D., Nguyen, C., Men, A., Hadler, J., Scott, P., Miyahara, A., Biswas, B., Carroll B., Chan, P. K., Hirani, T., Djordjevic, M. and Kinkema, M. (2007). Long distance regulation of plant stem cell growth during legume nodulation. In: ComBio 2007, Sydney, Australia, (). 22-26 September 2007.

Author Gresshoff P.M.
Kereszt, A.
Indrasumunar, A.
Nontachaiyapoom, S.
Lin, Y. H.
Jiang, Q.
Miyagi, M.
Oelkers, K.
Mathesius, U.
Lin, M.
Li, D.
Nguyen, C.
Men, A.
Hadler, J.
Scott, P.
Miyahara, A.
Biswas, B.
Carroll B.
Chan, P. K.
Hirani, T.
Djordjevic, M.
Kinkema, M.
Title of paper Long distance regulation of plant stem cell growth during legume nodulation
Conference name ComBio 2007
Conference location Sydney, Australia
Conference dates 22-26 September 2007
Publication Year 2007
Sub-type Published abstract
Language eng
Abstract/Summary Plants possess stem cells of immense pluripotency. The study of Rhizobium-induced proliferation of pericycle and cortical cells leading to nodule development involves activation of repressed stem cells. The amount of Rhizobium-induced symbiotic tissue in a legume is genetically controlled. The systemic 'Autoregulation of Nodulation' (AON) circuit (involving the LRR receptor kinase GmNARK) was further elucidated by expression and promoter analysis of GmNARK, and the development of a bioassay for the AON shoot-derived inhibitor (SDI). SDI was extracted, partially characterised, and re-fed to mutant plants, resulting in severe suppression of nodulation. Promoter analysis indicated (i) phloem localisation of GmNARK expression, (ii) a phloem expression cis-acting element also present in the orthologous Lotus japonicus Har1 andMedicago truncatula SUNN promoters, and (iii) functional interchangeability with the LjHar1 promoter. Two genes controlling the perception of the inducing Nod Factor were genetically verified. Increased nodulation, nitrogen gain and ability to nodulate effectively at sub-optimal Bradyrhizobium titres and environmental stress situation were achieved after overexpression of the Nod factor receptor gene (GmNFR1α). Cloning of GmNFR1 and GmNFR5, (the two putative components of the Nod factor receptor complex), the complementation of mutants, and nodulation efficiency analysis suggest that the AON circuit acts by perception of the NF signalling cascade and subsequently targeting its efficiency to regulate negatively the proliferation of plant stem cell.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
Collection: School of Agriculture and Food Sciences
 
Versions
Version Filter Type
Citation counts: Google Scholar Search Google Scholar
Created: Fri, 25 Feb 2011, 11:09:23 EST by Dr Arief Indrasumunar on behalf of Central Glasshouse Services