CDC25B associates with a centrin 2-containing complex and is involved in maintaining centrosome integrity

Boutros, Rose, Lorenzo, Corinne, Mondesert, Odile, Jauneau, Alain, Oakes,Vanessa, Dozier, Christine, Gabrielli, Brian and Ducommun, Bernard (2011) CDC25B associates with a centrin 2-containing complex and is involved in maintaining centrosome integrity. Biology of the Cell, 103 2: 55-68. doi:10.1042/BC20100111

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Author Boutros, Rose
Lorenzo, Corinne
Mondesert, Odile
Jauneau, Alain
Oakes,Vanessa
Dozier, Christine
Gabrielli, Brian
Ducommun, Bernard
Title CDC25B associates with a centrin 2-containing complex and is involved in maintaining centrosome integrity
Journal name Biology of the Cell   Check publisher's open access policy
ISSN 0248-4900
1768-322X
Publication date 2011-02-02
Year available 2010
Sub-type Article (original research)
DOI 10.1042/BC20100111
Volume 103
Issue 2
Start page 55
End page 68
Total pages 14
Place of publication London, United Kingdom
Publisher Portland Press Ltd.
Collection year 2011
Language eng
Formatted abstract
Background information.
CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin-dependent kinase)-cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers.

Results.
In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N- and C-terminal regions of CDC25B and requires CDC25B binding to its CDK-cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2.

Conclusions.
Our results therefore suggest that CDC25B associates with a Ctn2-containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components. © 2010 The Author(s).
Keyword CDC25B
Centrin (Ctn)
Centrosome
Cyclin-dependent kinase (CDK)
Fluorescence lifetime imaging microscopy (FLIM)
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
UQ Diamantina Institute Publications
 
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Created: Thu, 24 Feb 2011, 12:41:12 EST by Kylie Hengst on behalf of UQ Diamantina Institute