Duplicated nod-factor receptor 5 (NFR5) genes are mutated in soybean (Glycine max L. Merr.)

Gresshoff, P.M. and Indrasumunar, A. (2010) Duplicated nod-factor receptor 5 (NFR5) genes are mutated in soybean (Glycine max L. Merr.). Plant Signaling & Behavior, 5 5: 535-536.

Author Gresshoff, P.M.
Indrasumunar, A.
Title Duplicated nod-factor receptor 5 (NFR5) genes are mutated in soybean (Glycine max L. Merr.)
Journal name Plant Signaling & Behavior   Check publisher's open access policy
ISSN 1559-2316
Publication date 2010
Sub-type Other
Volume 5
Issue 5
Start page 535
End page 536
Total pages 2
Place of publication United States
Publisher Landes Bioscience
Collection year 2011
Language eng
Abstract In symbiosis with Bradyrhizobium japonicum, soybean (Glycine max L.) forms nitrogen-fixing nodules in its roots after mitogenic stimulation from a bacterial lipo-oligosaccharide (the 'Nodfactor'). In our recent paper in Plant and Cell Physiology we utilize two recessive loss-of-function plant mutants with a non-nodulation phenotype, and comparative genomics to clone and functionally analyze relevant soybean genes of the LysM receptor kinase family which are needed for perception of Nod-factor released by its microsymbiont B. japonicum. Two highly related lipo-oligochitin LysM type receptor kinase genes were cloned; they are presumed to be the critical nodulation inducing (Nod) factor receptor. These duplicated receptor genes were called GmNFR5α and GmNFR5β. Non-sense mutations in GmNFR5α and GmNFR5β were functionally complemented by both wild-type GmNFR5α and GmNFR5β in transgenic roots, indicating that both genes are functional. Both genes are wild-type in some soybean cultivars; however, non-functional NFR5β alleles were discovered in several others, which harbored an identical 1,407 bp retroelement-type insertion. GmNFR5α but not GmNFR5β was expressed in tap and lateral root portions at about 10-25% of GmATS1 (ATP synthase subunit 1), but not in trifoliate leaves and shoot tips. In general, inoculation treatment downregulated GmNFR5α/β transcripts in tap and lateral root portions. © 2010 Landes Bioscience.
Keyword Gene duplication
Nodulation
Receptor
Retroelement
Symbiosis
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Other
Collection: Centre for Integrative Legume Research Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 24 Feb 2011, 09:47:16 EST by Dr Arief Indrasumunar on behalf of Central Glasshouse Services