Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells

Yan, Y, Johnston, APR, Dodds, SJ, Kamphuis, MMJ, Ferguson, C, Parton, RG, Nice, EC, Heath, JK and Caruso, F (2010) Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells. ACS Nano, 4 5: 2928-2936. doi:10.1021/nn100173h


Author Yan, Y
Johnston, APR
Dodds, SJ
Kamphuis, MMJ
Ferguson, C
Parton, RG
Nice, EC
Heath, JK
Caruso, F
Title Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells
Journal name ACS Nano   Check publisher's open access policy
ISSN 1936-0851
1936-086X
Publication date 2010-05-25
Sub-type Article (original research)
DOI 10.1021/nn100173h
Volume 4
Issue 5
Start page 2928
End page 2936
Total pages 9
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2011
Language eng
Formatted abstract
Understanding the interactions between drug carriers and cells is of importance to enhance the delivery of therapeutics. The release of therapeutics into different intracellular environments, such as the lysosomes or the cell cytoplasm, will impact their pharmacological activity. Herein, we investigate the intracellular fate of layer-by-layer (LbL)-assembled, submicrometer-sized polymer hydrogel capsules in a human colon cancer derived cell line, LIM1899. The cellular uptake of the disulfide-stabilized poly(methacrylic acid) (PMASH) capsules by colon cancer cells is a time-dependent process. Confocal laser scanning microscopy and transmission electron microscopy reveal that the internalized capsules are deformed in membrane-enclosed compartments, which further mature to late endosomes or lysosomes. We further demonstrate the utility of these redox-responsive PMASH capsules for the delivery of doxorubicin (DOX) to colon cancer cells. The DOX-loaded PMASH capsules demonstrate a 5000-fold enhanced cytotoxicity in cell viability studies compared to free DOX.
© 2010 American Chemical Society.
Keyword Polymer capsules
Endocytosis
Intracellular fate
Colorectal cancer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 100 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 101 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 23 Feb 2011, 15:13:19 EST by Susan Allen on behalf of Institute for Molecular Bioscience