Metagenomic analysis of stressed coral holobionts

Thurber, Rebecca Vega, Willner-Hall, Dana, Rodriguez-Mueller, Christelle, Desnues, Christelle, Edwards, Robert A., Angly, Florent, Dinsdale, Elizabeth, Kelly, Linda and Rohwer, Forest (2009) Metagenomic analysis of stressed coral holobionts. Environmental Microbiology, 11 8: 2148-2163. doi:10.1111/j.1462-2920.2009.01935.x

Author Thurber, Rebecca Vega
Willner-Hall, Dana
Rodriguez-Mueller, Christelle
Desnues, Christelle
Edwards, Robert A.
Angly, Florent
Dinsdale, Elizabeth
Kelly, Linda
Rohwer, Forest
Title Metagenomic analysis of stressed coral holobionts
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
Publication date 2009-08
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2009.01935.x
Volume 11
Issue 8
Start page 2148
End page 2163
Total pages 16
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The coral holobiont is the community of metazoans, protists and microbes associated with scleractinian corals. Disruptions in these associations have been correlated with coral disease, but little is known about the series of events involved in the shift from mutualism to pathogenesis. To evaluate structural and functional changes in coral microbial communities, Porites compressa was exposed to four stressors: increased temperature, elevated nutrients, dissolved organic carbon loading and reduced pH. Microbial metagenomic samples were collected and pyrosequenced. Functional gene analysis demonstrated that stressors increased the abundance of microbial genes involved in virulence, stress resistance, sulfur and nitrogen metabolism, motility and chemotaxis, fatty acid and lipid utilization, and secondary metabolism. Relative changes in taxonomy also demonstrated that coral-associated microbiota (Archaea, Bacteria, protists) shifted from a healthy-associated coral community (e.g. Cyanobacteria, Proteobacteria and the zooxanthellae Symbiodinium) to a community (e.g. Bacteriodetes, Fusobacteria and Fungi) of microbes often found on diseased corals. Additionally, low-abundance Vibrio spp. were found to significantly alter microbiome metabolism, suggesting that the contribution of a just a few members of a community can profoundly shift the health status of the coral holobiont.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
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Created: Mon, 21 Feb 2011, 14:31:16 EST