Genomic analysis of multiple Roseophage SIO1 strains

Angly, F., Youle, M., Nosrat, B., Srinagesh, S., Rodriguez-Brito, B., McNairnie, P., Deyanat-Yazdi, G., Breitbart, M. and Rohwer, F. (2009) Genomic analysis of multiple Roseophage SIO1 strains. Environmental Microbiology, 11 11: 2863-2873. doi:10.1111/j.1462-2920.2009.02021.x


Author Angly, F.
Youle, M.
Nosrat, B.
Srinagesh, S.
Rodriguez-Brito, B.
McNairnie, P.
Deyanat-Yazdi, G.
Breitbart, M.
Rohwer, F.
Title Genomic analysis of multiple Roseophage SIO1 strains
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2009-11
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2009.02021.x
Volume 11
Issue 11
Start page 2863
End page 2873
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract P>Roseophage SIO1 is a lytic marine phage that infects Roseobacter SIO67, a member of the Roseobacter clade of near-shore alphaproteobacteria. Roseophage SIO1 was first isolated in 1989 and sequenced in 2000. We have re-sequenced and re-annotated the original isolate. Our current annotation could only assign functions to seven additional open reading frames, indicating that, despite the advances in bioinformatics tools and increased genomic resources, we are still far from being able to translate phage genomic sequences into biological functions. In 2001, we isolated four new strains of Roseophage SIO1 from California near-shore locations. The genomes of all four were sequenced and compared against the original Roseophage SIO1 isolated in 1989. A high degree of conservation was evident across all five genomes; comparisons at the nucleotide level yielded an average 97% identity. The observed differences were clustered in protein-encoding regions and were mostly synonymous. The one strain that was found to possess an expanded host range also showed notable changes in putative tail protein-coding regions. Despite the possibly rapid evolution of phage and the mostly uncharacterized diversity found in viral metagenomic data sets, these findings indicate that viral genomes such as the genome of SIO1-like Roseophages can be stably maintained over ecologically significant time and distance (i.e. over a decade and similar to 50 km).
Keyword Marine-environment
Sequence Alignment
Gene-transfer
Bacteria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 34 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 21 Feb 2011, 14:30:18 EST