A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment

Morrow, Benjamin J., Keddie, Daniel J., Gueven, Nuri, Lavin, Martin F. and Bottle, Steven E. (2010) A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment. Free Radical Biology and Medicine, 49 1: 67-76. doi:10.1016/j.freeradbiomed.2010.03.019

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Morrow, Benjamin J.
Keddie, Daniel J.
Gueven, Nuri
Lavin, Martin F.
Bottle, Steven E.
Title A novel profluorescent nitroxide as a sensitive probe for the cellular redox environment
Journal name Free Radical Biology and Medicine   Check publisher's open access policy
ISSN 0891-5849
Publication date 2010-07-01
Sub-type Article (original research)
DOI 10.1016/j.freeradbiomed.2010.03.019
Volume 49
Issue 1
Start page 67
End page 76
Total pages 10
Place of publication United States
Publisher Elsevier Inc.
Collection year 2011
Language eng
Abstract Changes to the redox status of biological systems have been implicated in the pathogenesis of a wide variety of disorders. Sensitive quantification of these changes has been developed using a novel fluorescent probe containing a redox-sensitive nitroxide moiety. As well as being able to selectively detect the superoxide radical in vitro, this method can measure overall changes to the cellular redox environment using flow cytometry on the basis of nitroxide reduction. The reversible nature of the probe's detection mechanism offers the unique advantage of being able to monitor redox changes in both oxidizing and reducing directions in real time. © 2010 Elsevier Inc.
Keyword Fluorescent probe
Free radicals
Nitroxide
Oxidative stress
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 36 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 21 Feb 2011, 23:47:21 EST by Caroline Irle on behalf of UQ Centre for Clinical Research