Increased expression of alpha-enolase in cervico-vaginal fluid during labour

Dellios, Nicole I., Lappas, Martha, Young, I. Ross, Palliser, Hannah K., Hirst, Jonathan J., Oliva, Karen, Ayhan, Mustafa and Rice, Gregory E. (2010) Increased expression of alpha-enolase in cervico-vaginal fluid during labour. European Journal of Obstetrics and Gynecology and Reproductive Biology, 153 1: 16-22. doi:10.1016/j.ejogrb.2010.06.014


Author Dellios, Nicole I.
Lappas, Martha
Young, I. Ross
Palliser, Hannah K.
Hirst, Jonathan J.
Oliva, Karen
Ayhan, Mustafa
Rice, Gregory E.
Title Increased expression of alpha-enolase in cervico-vaginal fluid during labour
Journal name European Journal of Obstetrics and Gynecology and Reproductive Biology   Check publisher's open access policy
ISSN 0301-2115
1872-7654
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.ejogrb.2010.06.014
Volume 153
Issue 1
Start page 16
End page 22
Total pages 7
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Objectives: The aim of this study was (i) to characterise differentially expressed proteins in cervico-vaginal fluid (CVF) at the time of preterm labour onset and (ii) to confirm these studies in human CVF samples taken from women before and during spontaneous labour. Study design: Preterm labour was induced in sheep (n = 5) via fetal dexamethasone infusion (1 mg/24 h). CVF samples were taken prior to dexamethasone infusion (0 h), 28 h after the start of dexamethasone infusion, and immediately prior to delivery. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differentially expressed proteins. For the human studies, paired CVF samples were taken 5-9 days before labour and during spontaneous labour onset (n = 7). Results: There was a 4.2-fold increase in α-enolase protein expression in sheep CVF during labour. Likewise, α-enolase protein expression was significantly increased during spontaneous human labour at term. Conclusions: Alpha-enolase is known to be bound to neutrophils and interact in the immune response, and thus may play a role in inflammation associated with human labour.
© 2010 Elsevier Ireland Ltd All rights reserved.
Keyword Alpha-enolase
Cervico-vaginal fluid
Human labour
Mass spectrometry
Ovine
Two-dimensional gel electrophoresis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Non HERDC
 
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Created: Fri, 18 Feb 2011, 10:54:54 EST by Caroline Irle on behalf of UQ Centre for Clinical Research