Quantitative assessment of brain volumes in fish: Comparison of methodologies

Ullmann, Jeremy F. P., Cowin, Gary and Collin, Shaun P. (2011) Quantitative assessment of brain volumes in fish: Comparison of methodologies. Brain, Behavior and Evolution, 76 3-4: 261-270. doi:10.1159/000321467

Author Ullmann, Jeremy F. P.
Cowin, Gary
Collin, Shaun P.
Title Quantitative assessment of brain volumes in fish: Comparison of methodologies
Journal name Brain, Behavior and Evolution   Check publisher's open access policy
ISSN 0006-8977
Publication date 2011-01
Year available 2010
Sub-type Article (original research)
DOI 10.1159/000321467
Volume 76
Issue 3-4
Start page 261
End page 270
Total pages 10
Place of publication Basel, Switzerland
Publisher S. Karger AG
Collection year 2011
Language eng
Formatted abstract
When correlating brain areas with behavioral and environmental characteristics, a variety of techniques are employed. In fishes (elasmobranchs and teleosts), 2 methods, histology and the idealized ellipsoid and/or half-ellipsoid technique, are primarily used to calculate the volume of a brain area and therefore its relationship to social or ecological complexity. In this study on a perciform teleost, we have quantitatively compared brain volumes obtained using the conventional techniques of histology and approximating brain volume to an idealized ellipsoid (or half ellipsoid) and magnetic resonance imaging, an established clinical tool typically used for assessing brain volume in other vertebrates. Our results indicate that, when compared to brain volumes measured using magnetic resonance imaging of brain regions in situ, variations in brain shape and histological artifacts can lead to significant differences in brain volume, especially in the telencephalon and optic tecta. Consequently, in comparative studies of brain volumes, we advise caution when using the histological and/or ellipsoid methods to make correlations between brain area size and environmental, behavioral and social characteristics and, when possible, we propose the use of magnetic resonance imaging.
Keyword Brain morphology
Brain size
Ellipsoid technique
Comparative neuroanatomy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: November 16, 2010.

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Created: Mon, 14 Feb 2011, 15:46:06 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences