This study evaluated the biological and chemical quality of the water produced by a full-scale reclamation plant based on the combination of ozonation and activated carbon filtration. The biological quality of the final water was assessed by tests to detect the presence of pathogens. The chemical quality was assessed by the quantification of micropollutants (pharmaceuticals and pesticides) along the treatment train. Moreover, bioassays were used as complementary tools to evaluate the level of various biological adverse effects induced by the water. The results suggest that it is possible to produce reclaimed water of a quality comparable to reverse osmosis treated water without the problem of disposal of the concentrate stream.