Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster

Yamada, R., Iturbe-Ormaetxe, I., Brownlie, J. C. and O'Neill, S. L. (2011) Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster. Insect Molecular Biology, 20 1: 75-85. doi:10.1111/j.1365-2583.2010.01042.x


Author Yamada, R.
Iturbe-Ormaetxe, I.
Brownlie, J. C.
O'Neill, S. L.
Title Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster
Journal name Insect Molecular Biology   Check publisher's open access policy
ISSN 0962-1075
1365-2583
Publication date 2011-02
Year available 2010
Sub-type Article (original research)
DOI 10.1111/j.1365-2583.2010.01042.x
Volume 20
Issue 1
Start page 75
End page 85
Total pages 11
Place of publication Oxford , United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Abstract Wolbachia are inherited intracellular bacteria that infect a broad range of invertebrate hosts. They commonly manipulate host reproduction in a variety of ways and thereby favour their invasion into host populations. While the biology of Wolbachia has been extensively studied at the ecological and phenotypic level, little is known about the molecular mechanisms underlying the interaction between Wolbachia and their hosts. Recent comparative genomics studies of Wolbachia strains have revealed putative candidate genes involved in the expression of cytoplasmic incompatibility (CI) in insects. However the functional testing of these genes is hindered by the lack of available genetic tools in Wolbachia. To circumvent this problem we generated transgenic Drosophila lines expressing various Wolbachia CI candidate genes under the control of the GAL4/UAS system in order to evaluate their possible role in Wolbachia-related phenotypes in Drosophila. The expression of a number of these genes in Drosophila melanogaster failed to mimic or alter CI phenotypes across a range of Wolbachia backgrounds or in the absence of Wolbachia.
Keyword Cytoplasmic incompatibility
Wolbachia
Drosophila melanogaster
Transgenic fly
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 21 SEP 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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Created: Sun, 13 Feb 2011, 00:03:22 EST