A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata:Agamidae)

Ezaz, Tariq, O'Meally, Denis, Quinn, Alexander E., Sarre, Stephen D., Georges, Arthur and Marshall Graves, Jennifer A. (2008) A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata:Agamidae). Cytotechnology, 58 3: 135-139. doi:10.1007/s10616-009-9182-3


Author Ezaz, Tariq
O'Meally, Denis
Quinn, Alexander E.
Sarre, Stephen D.
Georges, Arthur
Marshall Graves, Jennifer A.
Title A simple non-invasive protocol to establish primary cell lines from tail and toe explants for cytogenetic studies in Australian dragon lizards (Squamata:Agamidae)
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
1573-0778
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1007/s10616-009-9182-3
Volume 58
Issue 3
Start page 135
End page 139
Total pages 5
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Formatted abstract
Primary cell lines were established from cultures of tail and toe clips of five species of Australian dragon lizards: Tympanocryptis pinguicolla, Tympanocryptis sp., Ctenophorus fordi, Amphibolurus norrisi and Pogona vitticeps. The start of exponential cell growth ranged from 1 to 5 weeks. Cultures from all specimens had fibroblastic morphology. Cell lines were propagated continuously up to ten passages, cryopreserved and recovered successfully. We found no reduction in cell viability after short term (<6 months) storage at -80 °C. Mitotic metaphase chromosomes were harvested from these cell lines and used in differential staining, banding and fluorescent in situ hybridisation. Cell lines maintained normal diploidy in all species. This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice. The method is likely to be applicable to a range of species. Such cell lines provide a virtually unlimited source of material for cytogenetic, evolutionary and genomic studies.
© 2009 Springer Science+Business Media B.V.
Keyword Reptiles
Lizards
Toe
Non-invasive
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Published under Brief Report

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
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Created: Fri, 11 Feb 2011, 15:33:56 EST by Susan Allen on behalf of Institute for Molecular Bioscience