The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation

Borngen, K, Battle, AR, Moker, N, Morbach, S, Marin, K, Martinac, B and Kramer, R (2010) The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1798 11: 2141-2149. doi:10.1016/j.bbamem.2010.06.022


Author Borngen, K
Battle, AR
Moker, N
Morbach, S
Marin, K
Martinac, B
Kramer, R
Title The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation
Formatted title
The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation
Journal name Biochimica et Biophysica Acta (BBA) - Biomembranes   Check publisher's open access policy
ISSN 0005-2736
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.bbamem.2010.06.022
Volume 1798
Issue 11
Start page 2141
End page 2149
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Based on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior. Apart from that, MscCG is characterized by significant functional differences with respect to conductance, ion selectivity and desensitation behavior as compared to MscS from E. coli. Deletion and complementation studies in C. glutamicum showed a significant contribution of MscCG to betaine efflux in response to hypoosmotic conditions. A detailed analysis of concomitant betaine uptake (by the betaine transporter BetP) and efflux (by MscCG) under hyperosmotic conditions indicates that MscCG may act in osmoregulation in C. glutamicum by fine-tuning the steady state concentration of compatible solutes in the cytoplasm which are accumulated in response to hyperosmotic stress. © 2010 Elsevier B.V.
Keyword Mechanosensitive channel
Corynebacterium
Efflux
Osmoregulation
Uptake
Betaine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Created: Thu, 10 Feb 2011, 17:14:30 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences