Abundant transcripts of malting barley identified by serial analysis of gene expression (SAGE)

White, Jessica, Pacey-Miller, Toni, Crawford, Allison, Cordeiro, Glovanni, Barbary, Daniel, Bundock, Peter and Henry, Robert (2006) Abundant transcripts of malting barley identified by serial analysis of gene expression (SAGE). Plant Biotechnology Journal, 4 3: 289-301.


Author White, Jessica
Pacey-Miller, Toni
Crawford, Allison
Cordeiro, Glovanni
Barbary, Daniel
Bundock, Peter
Henry, Robert
Title Abundant transcripts of malting barley identified by serial analysis of gene expression (SAGE)
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
1467-7652
Publication date 2006-05
Sub-type Article (original research)
DOI 10.1111/j.1467-7652.2006.00181.x
Volume 4
Issue 3
Start page 289
End page 301
Total pages 13
Place of publication Oxford, England, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract Serial analysis of gene expression (SAGE) was applied to the major cereal crop barley (Hordeum vulgare) to characterize the transcriptional profile of grain during the malting process. Seven SAGE libraries were generated from seed at different time points during malting, in addition to one library from dry mature seed. A total of 155 206 LongSAGE tags, representing 41 909 unique sequences, was generated. This study reports an in-depth analysis of the most abundant transcripts from each of eight specific time points in a malting barley time course. The 100 most abundant tags from each library were analysed to identify the putative functional role of highly abundant transcripts. The largest functional groups included transcripts coding for stress response and cell defence, ribosomal proteins and storage proteins. The most abundant tag represented B22EL8, a barley metallothionein, which showed significant up-regulation across the malting time course. Considerable changes in the abundance profiles of some of the highly abundant tags occurred at 24 h post-steeping, indicating that it may be an important time point for gene expression changes associated with barley seed germination. © 2006 Blackwell Publishing Ltd.
Keyword Germination
Hordeum vulgare
Seed
Serial analysis of gene expression (SAGE)
Transcript
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
ERA 2012 Admin Only
 
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