An improved procedure for the methylation analysis of oligosaccharides and polysaccharides

Harris, Philip J., Henry, Robert J., Blakeney, Anthony B. and Stone, Bruce A. (1984) An improved procedure for the methylation analysis of oligosaccharides and polysaccharides. Carbohydrate Research, 127 1: 59-73.


Author Harris, Philip J.
Henry, Robert J.
Blakeney, Anthony B.
Stone, Bruce A.
Title An improved procedure for the methylation analysis of oligosaccharides and polysaccharides
Journal name Carbohydrate Research   Check publisher's open access policy
ISSN 0008-6215
1873-426X
Publication date 1984-04-02
Sub-type Article (original research)
DOI 10.1016/0008-6215(84)85106-X
Volume 127
Issue 1
Start page 59
End page 73
Total pages 15
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Language eng
Formatted abstract An improved procedure for the methylation analysis of oligosaccharides and polysaccharides is described. Steps in the procedure were examined and optimised for quantitative recovery and speed. Methylation was shown to be complete by using [14C]methyl iodide. All operations were performed in the same tube and the need to concentrate solutions containing acetylated alditols of methylated sugars was eliminated, thus minimising losses due to volatilization. The method is convenient, gives high recoveries of acetylated alditols of methylated sugars, and allows analysis of the glycosyl linkages of oligo- or poly-saccharides to be completed within a working day. A wide range of oligo- and poly-saccharides were methylated by this procedure.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 459 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 234 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 55 Abstract Views  -  Detailed Statistics
Created: Wed, 09 Feb 2011, 12:07:11 EST