A perfect marker for fragrance genotyping in rice

Bradbury, Louis M. T., Henry, Robert J., Jin, Qingsheng, Reinke, Russell F. and Waters, Daniel L. E. (2005) A perfect marker for fragrance genotyping in rice. Molecular Breeding, 16 4: 279-283. doi:10.1007/s11032-005-0776-y

Author Bradbury, Louis M. T.
Henry, Robert J.
Jin, Qingsheng
Reinke, Russell F.
Waters, Daniel L. E.
Title A perfect marker for fragrance genotyping in rice
Journal name Molecular Breeding   Check publisher's open access policy
ISSN 1380-3743
Publication date 2005-11
Sub-type Article (original research)
DOI 10.1007/s11032-005-0776-y
Volume 16
Issue 4
Start page 279
End page 283
Total pages 5
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Formatted abstract
Allele specific amplification (ASA) is a low-cost, robust technique that can be utilised to discriminate between alleles that differ by SNP's, insertions or deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has been shown to be due to an eight bp deletion and three SNP's in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2). Here we report a single tube ASA assay which allows discrimination between fragrant and non-fragrant rice varieties and identifies homozygous fragrant, homozygous non-fragrant and heterozygous non-fragrant individuals in a population segregating for fragrance. External primers generate a fragment of approximately 580 bp as a positive control for each sample. Internal and corresponding external primers produce a 355 bp fragment from a non-fragrant allele and a 257 bp fragment from a fragrant allele, allowing simple analysis on agarose gels.
Keyword aromatic
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
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Created: Wed, 09 Feb 2011, 12:01:21 EST