Widespread expression of the mRNA encoding a novel vitamin D/thyroxine dual binding protein in the turtle Trachemys scripta

Whitworth, Deanne J., Hunt, Leigh and Licht, Paul (2000) Widespread expression of the mRNA encoding a novel vitamin D/thyroxine dual binding protein in the turtle Trachemys scripta. General and Comparative Endocrinology, 118 2: 354-358.

Author Whitworth, Deanne J.
Hunt, Leigh
Licht, Paul
Title Widespread expression of the mRNA encoding a novel vitamin D/thyroxine dual binding protein in the turtle Trachemys scripta
Formatted title
Widespread expression of the mRNA encoding a novel vitamin D/thyroxine dual binding protein in the turtle Trachemys scripta
Journal name General and Comparative Endocrinology   Check publisher's open access policy
ISSN 0016-6480
1095-6840
Publication date 2000-05
Sub-type Article (original research)
Volume 118
Issue 2
Start page 354
End page 358
Total pages 5
Place of publication Maryland Heights, MO, U.S.A.
Publisher Academic Press
Language eng
Formatted abstract
Vitamin D-binding protein (DBP) is the major transport protein for the vitamin D sterols in a variety of mammalian and avian species. The DBP found in the emydid family of turtles is unique in that it exhibits high-affinity binding of both vitamin D and thyroxine (D/TBP). Sequence analysis has revealed that the emydid D/TBP is homologous to mammalian DBP and shares no homology with mammalian thyroxine-binding globulins. Northern blot analysis was used to examine the spatial profile of D/TBP transcription in the turtle Trachemys scripta. In both adults and hatchlings, two transcripts—the expected 1.5-kb full-length transcript and a second 0.8-kb transcript—were present in nearly all of the tissues examined. In adults, highest expression of the 1.5-kb transcript was seen in the kidney, gonad, and spleen, with lower levels in the liver and lung and no transcripts in skeletal muscle. In hatchlings, the full-length transcript was detected in a variety of tissues at similar levels. Injection of hatchlings with thyroxine increased levels of circulating D/TBP and transcript levels. These data are in marked contrast to observations in mammals in which transcription of DBP is confined predominantly to the liver. Further, the increase in circulating D/TBP associated with increased thyroidal activity may result from a direct or indirect activation of D/TBP transcription by thyroxine.
Keyword Vitamin D-binding protein
Thyroxine-binding protein
Northern blotting
Reptile
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 09 Feb 2011, 11:11:23 EST by Dr Deanne Whitworth on behalf of School of Veterinary Science