Determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography

Zhu, J., Ng, J. and Filippich, L. J. (1992) Determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography. Journal of Chromatography B, 577 1: 77-85. doi:10.1016/0378-4347(92)80600-U


Author Zhu, J.
Ng, J.
Filippich, L. J.
Title Determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography
Journal name Journal of Chromatography B
ISSN 0378-4347
1873-376X
Publication date 1992-05
Sub-type Article (original research)
DOI 10.1016/0378-4347(92)80600-U
Volume 577
Issue 1
Start page 77
End page 85
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A method for the identification and determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography was developed. Tannic acid and four phenolic compounds, namely gallic acid, pyrogallol, 4-O-methylgallic acid and ellagic acid, were successfully extracted from the biological fluids by using ethyl acetate at acidic conditions. Gallic acid, pyrogallol and 4-O-methylgallic acid were found in the sheep urine, gallic acid, 4-O-methylgallic acid and ellagic acid in plasma, and gallic acid and ellagic acid in abomasal fluid after abomasal dosing of tannic acid. Tannic acid was found in the plasma apart from the abomasal fluid into which it was administered. The concentrations of tannic acid, gallic acid, pyrogallol, 4-O-methylgallic acid and ellagic acid in plasma, abomasal fluid and urine were measured. This method could be applied to measurement of other hydrolysable tannins and their phenolic metabolites in bioligical materials.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: National Research Centre for Environmental Toxicology Publications
 
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Created: Wed, 09 Feb 2011, 10:53:08 EST