The stressosome: Molecular architecture of a signalling hub

Marles-Wright, Jon and Lewis, Richard J. (2010) The stressosome: Molecular architecture of a signalling hub. Biochemical Society Transactions, 38 4: 928-933. doi:10.1042/BST0380928


Author Marles-Wright, Jon
Lewis, Richard J.
Title The stressosome: Molecular architecture of a signalling hub
Journal name Biochemical Society Transactions   Check publisher's open access policy
ISSN 0300-5127
1470-8752
Publication date 2010-08
Year available 2010
Sub-type Article (original research)
DOI 10.1042/BST0380928
Volume 38
Issue 4
Start page 928
End page 933
Total pages 6
Place of publication Colchester, U.K.
Publisher Portland Press
Collection year 2011
Language eng
Formatted abstract
The stressosome co-ordinates the response of Bacillus subtilis to the imposition of a variety of physical and environmental insults. These stresses include fluctuations in salt concentration, the presence of ethanol, changes in pH and even the level of UV light. Despite the obvious and significant differences between these quite different physicochemical stimuli, the result is the same: the stressosome is phosphorylated by a key kinase to initiate the σB cascade. The phosphorylation of the stressosome initiates a signal transduction system that up-regulates the expression of stress-responsive genes so that the Bacillus can survive the imposition of stress. Hence the stressosome acts as a hub, receiving manifold different stimuli to effect a single outcome. Using single-particle analysis of cryo-electron micrographs, we have been able to reconstruct a series of molecular envelopes of the stressosome. These maps have been interpreted at near-atomic resolution with crystal structures of the individual components of the stressosome to provide the first visualization of this unique signalling hub. The macromolecular structure adopted by the stressosome provides the signalling cascade with the potential for co-operative behaviour, which we have also measured in live bacteria. These experiments are consistent with the tuning of the response of B. subtilis to stress relative to the magnitude of the insult.
©The Authors Journal compilation ©2010 Biochemical Society
Keyword Bacillus subtilis
Kinase
Signal transduction
Stress response
Stressosome
Structural biology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 08 Feb 2011, 10:44:44 EST by Susan Allen on behalf of !NON-HERDC